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GHK-Cu pretreatment showed a dose-dependent trend towards lower MPO activity and neutrophil infiltration
The antioxidant solution was prepared as follows: a stock solution containing 4% ascorbic acid (VWR, Leuven, Belgium), 0.4% glutathione and 2% uric acid (Sigma-Aldrich, Lyon, France) was prepared in distilled water and filtered using a 0.2 m filter after adjusting the pH to 7.5 with 10 M KOH
However, the larger ACT trial (n=2,308) did not confirm this benefit (ACT Investigators, Circulation, 2011) [68]
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