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Discussion The original method faced several challenges, including the need for strong acids to terminate the enzymatic reaction, which could be hazardous and affect the accuracy of the measurement

(2) Bioavailability challenges : Many active ingredients of TCM exhibited suboptimal pharmacokinetic profiles due to structural instability, rapid oxidation, and poor membrane permeability, advanced delivery systems (e.g., lipid-based encapsulation, nanoparticle carriers) can be considered

Experimental data have already demonstrated that over 70 percent of the expressed proteins in spirulina remained intact after two hours of incubation under simulated gastric conditions that would fully degrade the same protein in a purified form within minutes