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2019;51(6):e13267

Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken

Molfino A, Cascino A, Conte C, Ramaccini C, Rossi Fanelli F, Laviano A: Caloric restriction and L-carnitine administration improves insulin sensitivity in patients with impaired glucose metabolism