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qRTPCR was performed via SYBR Premix Ex Taq II (Takara) on a Chromo 4 continuous fluorescence detector with a PTC-200 DNA Engine Cycler (Bio-Rad Laboratories, Hercules, CA)
In the absence of safety data, most clinicians advise that pregnant or breastfeeding individuals avoid this compounded preparation unless the anticipated benefit clearly outweighs theoretical fetal or neonatal risks
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