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Diabetes Care 37 , 30163024 (2014)
2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions
Both glucose and sodium ions bind to the cotransport protein, with the Na + electrochemical gradient, maintained by the basolateral sodiumpotassium adenosine triphosphatase, driving glucose uptake across the luminal membrane ( Figure 1 )
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