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These concerns were later addressed by using separate fibers for capillaries and venules and replacing astrocytes with human smooth muscle cells (Cucullo et al., Even with these advantages, DIV-BBB models were superseded by BBB-on-chip models based on nano-fabrication and microfluidic techniques (Ma et al., 2005)

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Creating a physiological cerebrovasculature in vitro and understanding its disruption in different pathologies have been a major challenge

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